3.4.21.38 - 95409, 95414 3.4.21.38 FXII gene sequence determination, genotyping 707841 3.4.21.38 genotyping, determination and analysis of mutant K346N gene sequence, transient expression of wild-type and K346N mutant FXII in CHO-K1 cells. Quantitative RT-PCR FXII mRNA expression analysis 710605 3.4.21.38 the cDNA of the zymogen domain of FXII is cloned into the pET-28a(+) vector and the plasmid is transformed into Escherichia coli strain BL21 (DE3) and overexpressed 653755 3.4.21.38 the FXII promoter contains the -299/+1 region, that is sufficient for TGF-beta1 to induce FXII expression 709089