3.4.17.23	-	755534	
3.4.17.23	30 M HEK293 Freestyle cells are transfected with 293fectin combined with 30 mg of pFuse-based vectors containing the ACE2 construct	762712	
3.4.17.23	ACE2 expressed in Chinese hamster ovary cells specifically binds to glutathione-S-transferase-calmodulin, but not glutathione-S-transferase alone	692094	
3.4.17.23	ACE2 expression analysis	710214	
3.4.17.23	ACE2 expression analysis by RT-PCR	708195	
3.4.17.23	cloning and expression of a constitutively secreted form of ACE2, WKY rats are transduced with lentiviral vector containing shACE2. The plasma ACE2 levels could be increased by lentivector-mediated shACE2 gene transfer. This provides a tool to investigate the role of this enzyme in the development of the cardiovascular disease both through the role of hyperactivity of the RAS and through infectious agents	670861	
3.4.17.23	cloning of the enzyme utilizing the murine cytomegalovirus immediate early gene promoter, MCMV Pr, in an adenoviral vector for ACE2 overexpression in rats as a gene therapy model. overview	708649	
3.4.17.23	development of a transgenic mouse model (syn-hACE2) where the full open reading frame of the human ACE2 gene is under the control of a synapsin promoter, allowing the hACE2 protein to be expressed specifically in neurons	693733	
3.4.17.23	DNA and amino acid sequence determination, gene maps to choromosomal location Xp22, expression in CHO cells of the wild-type and of the soluble truncated mutant, the latter as c-Myc- and His-tagged protein	659147	
3.4.17.23	expressed in CHO cells	681678	
3.4.17.23	expressed in Escherichia coli BL21(DE3) cells	682824	
3.4.17.23	expressed in HEK 293-T cells	679708	
3.4.17.23	expressed in HEK-ACE2 cells	679876	
3.4.17.23	expressed in the endothelial cell line Eahy926	678973	
3.4.17.23	expression in CHO cells	707055	
3.4.17.23	expression in CHO cells and polarized Madin-Darby canine kidney epithelial cells	669404	
3.4.17.23	expression in E4 cells	707055	
3.4.17.23	expression in HEK-293 cells	692319	
3.4.17.23	expression in Spodoptera frugiperda Sf9 cells via infection with baculovirus	659172	
3.4.17.23	expression in the avian fibroblast cell line, DF1	765871	
3.4.17.23	expression of a fusion of ACE2 to the Fc domain of IgG1	764302	
3.4.17.23	expression of extracellular domain and wild-type, both as FLAG-tagged proteins, in Spodotera frugiperda Sf9 cells via baculovirus infection	659198	
3.4.17.23	expression of recombinant ACE2 in P-selectin-transfected Chinese hamster ovary cells	668418	
3.4.17.23	expression of the extracellular domain of human ACE2, residues Gln18-Ser740	763895	
3.4.17.23	expression of the mutant enzyme in CHO cells	657967	
3.4.17.23	expression of wild-type and utant L584A ACE2 in HEK-293 cells	707074	
3.4.17.23	gene ACE2, DNA sequence determination and analysis, expression in CHO K1 cells, secretion of the active enzyme from transfected cells by cleavage N-terminal to the transmembrane domain	658455	
3.4.17.23	N-terminal SNAP-tagged human ACE2 labeled with terbium, expression in EK-293 cell	764432	
3.4.17.23	overexpression of ACE2, by usage of a recombinant adeno-associated virus 6 delivery system, in myocardium of stroke-prone spontaneously hypertensive rats, gene expression profiling, overview	708684	
3.4.17.23	Sf21 cells via infection with baculovirus, mIgG-tagged protein	657531