3.2.1.94 - 3.2.1.94 expressed in Bacillus subtilis 3.2.1.94 expressed in Escherichia coli 3.2.1.94 expression in Escherichia coli 3.2.1.94 expression in Escherichia coli BL21(DE3) 3.2.1.94 the signal peptide of of Arthrobacter globiformis isomaltodextranase containing the twin-arginine motif is fused with GFP, and the corresponding plasmid pPIGFP is transformed into the wild type, Corynebacterium glutamicum ATCC 1386. The signal peptide of IMD is a bona fide Tat pathway targeting signal