3.2.1.62 COS-1 cells triple transfected with LPH–CFP, SI–YFP and Rab4, Rab8 or Rab11 fused to DsRed. MDCK cells stably expressing LPHmyc 701338 3.2.1.62 expressed in CACO-2 cells 699936 3.2.1.62 expressed in Escherichia coli BL21(DE3) cells 732073, 749451, 750706 3.2.1.62 expressed in Escherichia coli BL21(DE3) pLysS cells 749905 3.2.1.62 expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen 680835 3.2.1.62 expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen, in HEK293 cells cotransfected with in KlrP siRNA, overexpression in Escherichia coli 680835 3.2.1.62 expression in Cos-1 cells 208745, 208747 3.2.1.62 expression of mutants in Cos-7 cells 208744 3.2.1.62 expression of the gene lacking the 30-residue N-terminal secretion signal sequence in Escherichia coli, selenomethionine-derivatized enzyme 680770 3.2.1.62 genotyping 709206 3.2.1.62 LPH 5' upstream subcloned from rat liver genomic DNA 699936 3.2.1.62 mutant G1363S introduced into the wild-type LPH cDNA cloned in the vector pSG5. Mutant transiently expressed in COS-1 cells 698078 3.2.1.62 transient expression of wild-type and domain deletion mutant enzymes and isolated enzyme domains in Cos-1 cells, expression in MDCK cells 709142