3.2.1.153 48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 3.2.1.153 enzyme expression analysis of isozymes under different water conditions, overview 3.2.1.153 expressed in Pichia pastoris 3.2.1.153 expression in Pichia pastoris 3.2.1.153 fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb 3.2.1.153 gene 1-FEH1, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEH2a, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEH2b, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEHa, quantitative RT-PCR expression analysis 3.2.1.153 gene FEH-1, real-time PCR quantitative expression analysis 3.2.1.153 gene FEH-I, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 3.2.1.153 gene FEH-IIa, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 3.2.1.153 gene FEH-IIb, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 3.2.1.153 gene Wfh-sm3, recombinant expression in Pichia pastoris 3.2.1.153 heterologous expression in Pichia pastoris strain X-33 after culturing of plasmids in Escherichia coli, expression vector pPICZalphaA 3.2.1.153 heterologous expression in Pichia pastoris, pPICZalphaA vector 3.2.1.153 isoenzyme 1-FEH w1 3.2.1.153 isoenzyme 1-FEH w2 3.2.1.153 quantitative real time PCR analysis of the FEH mRNAs (including FEHI and FEHII) in cold stored roots of three chicory cultivars, overview. Variable levels of FEHII and FEHI expression in different phenotypes of the chicory cultivars 3.2.1.153 single copy gene Bp1-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Pichia pastoris