3.2.1.135	all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli	
3.2.1.135	Bifidobacterium breve cultured in reinforced clostridial medium, anaeorbic, 37°C. Escherichia coli cultured in Luria Bertani broth, agitated, 37°C. Lactococcus lactis cultured in M17 broth containing 0.5% glucose, 30°C for amplification of vectro pNZ8048 containing only alpha-amylase or pullulanase encoding domains, respectively. Selection of recombinant Escherichia coli with pORI19-apuB on agar containing erythromycin and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside and isopropyl-beta-D-galactopyranoside	
3.2.1.135	development of an an hydrotetracycline-inducible expression system for expression of a neopullulanase in Bacillus subtilis	
3.2.1.135	Escherichia coli DH5alphaF' grown in Luria-Bertani broth, 37°C with plasmid pTZ57R/T, expression in yeast Pichia pastoris with recombinant plasmid pPICZ-BK193 in Yeast Extract Peptone Dextrose	
3.2.1.135	Escherichia coli NovaBlue and Rosetta (DE3) pLysS, cloning and expression host, respectively, with plasmid pET-20b(+), in LB broth, 37°C	
3.2.1.135	expression in Bacillus subtilis	
3.2.1.135	expression in bacteria	
3.2.1.135	expression in Escherichia coli	
3.2.1.135	expression in Escherichia coli BL21 (DE3)	
3.2.1.135	expression in Escherichia coli CodonPlus BL21 (DE3)	
3.2.1.135	expression in Escherichia coli. A poly-His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellular uneven distribution in vivo in the cytoplasm of Escherichia coli	
3.2.1.135	expression in Saccharomyces cerevisiae	
3.2.1.135	NCL21 with plasmid pRN-PulSa, pRN-Puldelta3