3.1.4.46	-	
3.1.4.46	13 potential homologues are identified and subdivided into two groups: the first comprising proteins with only one GP-PDE domain or canonical type A enzymes, AtGDPD1-6, and the second including proteins with two putative GP-PDE domains, type B enzymes AtGDPDL1-7	
3.1.4.46	336 amino acids	
3.1.4.46	complete coding sequence by using genomic DNA of strain FR64B, 334 amino acids	
3.1.4.46	constitutive enzyme, transgenic enzyme expression can complement the enzyme UgpQ in a gene ugpQ-deleted strain of Escherichia coli	
3.1.4.46	expressed as His-tag fusion protein in Escherichia coli TOP10 cells	
3.1.4.46	expressed in Escherichia coli	
3.1.4.46	expressed in Escherichia coli BL21(DE3)	
3.1.4.46	expressed in Escherichia coli DH5alpha	
3.1.4.46	expressin of Myc-tagged vectors in COS-7 cells	
3.1.4.46	expression in Escherichia coli	
3.1.4.46	expression in Escherichia coli DL41 with His-Tag	
3.1.4.46	GDE2, maps to 11q13.4-13.5, and contains 17 exons and 16 introns, overexpression of a the tagged GDE2 in COS-7, HEK-293cells, and in HeLa cell endoplasmic reticulum, as well as at the plasma membrane, depending on cell confluence, with a predominant plasma-membrane localization in confluent	
3.1.4.46	gene glpQ, phylogenetic analysis	
3.1.4.46	gene gpdQ, DNA and amino acid seuence determination and analysis	
3.1.4.46	gene ugpQ, phylogenetic analysis	
3.1.4.46	gene YPL110c	
3.1.4.46	gene YPL206c	
3.1.4.46	generation of an 86-028NP-derived NTHI strain that expresses an hpd/glpQ promoter-driven luciferase reporter from the vector pKMLN-01, hpd/glpQ is expressed in an operon with glpT	
3.1.4.46	isolation of cDNAs encoding GDE2 and GDE6, cloned by using mouse brain cDNA libraries, expression in Escherichia coli JM109	
3.1.4.46	protein in Escherichia coli BL21(DE3)	
3.1.4.46	the genome encodes seven genes, glpQ1-3 and ugpQ1-4	
3.1.4.46	YqiK is regulated by the same operon yqiHIK as other hydrolytic enzymes