3.1.1.79 - 656446, 751513 3.1.1.79 cloned and expressed as a His-tagged fusion protein in Escherichia coli 706584 3.1.1.79 cloning of cDNA 654418 3.1.1.79 expressed in 293A cells 690756 3.1.1.79 expressed in Escherichia coli 703845 3.1.1.79 expressed in THP-1- cells 690756 3.1.1.79 expression in CHO cells 654564 3.1.1.79 expression in COS and insect cells 655415 3.1.1.79 expression in Escherichia coli 677872, 750496, 752198 3.1.1.79 expression in Sf9 cell 750039 3.1.1.79 expression in Sf9 cells 750035 3.1.1.79 expression in Sf9 insect cells and COS cells 654572 3.1.1.79 expression of cDNA in COS cells 655533 3.1.1.79 expression of HSL cDNA in HepG2 cells 654476 3.1.1.79 expression of the entire enzyme, N-terminally and C-terminally His6-tagged, as a fully soluble protein in Escherichia coli in the presence of either the osmolyte trehalose, plus high salt concentration, or the membrane fluidizer benzyl alcohol. Addition of these compounds proves to be decisive in procuring a high expression level of stable, soluble, full-length recombinant protein 729130 3.1.1.79 expression Sf9 insect cells 654765, 657233 3.1.1.79 five C-terminally His-tagged human HSL variants are successfully cloned and expressed in Sf9 insect cells using the baculovirus/insect cell expression system 694826 3.1.1.79 gene est06, screening of a forest soil metagenomic library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, esterase (Est06) belonging to the hormone- sensitive lipase family of lipolytic enzymes is subcloned and heterologously expressed in Escherichia coli strain BL21(DE3) 750161 3.1.1.79 gene Est25, phylogenetic analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain XL1-Blue 728869 3.1.1.79 gene Lipe, encodes a specific HSL isoform including a 313 amino acid N-terminus encoded by the testis-specific exon T1 729643 3.1.1.79 gene MGL_0797, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 751503 3.1.1.79 gene MGL_0799, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 751503 3.1.1.79 gene MGL_4054, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 751503 3.1.1.79 gene MGL_4197, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 751503 3.1.1.79 heart-specific HSL-overexpressing mice, transgenic (Tg) mice with heart-specific HSL overexpression are generated, and cardiac histology, function, lipid profile, and gene expressions are analyzed after induction of diabetes by streptozotocin. HSL overexpression inhibits cardiac steatosis in streptozotocin (STZ)-induced diabetic mice 690342 3.1.1.79 human HSL is expressed from baculovirus-infected insect cells 714319 3.1.1.79 isozyme HSL1, DNA and amino acid sequence determination and analysis, sequence comparison of HSL1 and HSL2, and phylogenetic analysis, quantitative real-time PCR expression analysis 714766 3.1.1.79 isozyme HSL2, DNA and amino acid sequence determination and analysis, sequence comparison of HSL1 and HSL2, and phylogenetic analysis, quantitative real-time PCR expression analysis 714766 3.1.1.79 overexpressed in BL21(DE3) cells 690779 3.1.1.79 recombinant expression of His-tagged enzyme 730002 3.1.1.79 sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha 750177