3.1.1.41 expressed in Acremonium chrysogenum 675216 3.1.1.41 expressed in Escherichia coli DH5alpha cells 749261 3.1.1.41 expressed in Escherichia coli JM109 671502 3.1.1.41 expression in Escherichia coli 170674, 170677, 752071 3.1.1.41 expression in Escherichia coli BL21 747164 3.1.1.41 gene axe, expression of His-tagged enzyme in Escherichia coli 677538 3.1.1.41 gene Cah, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3), the enzyme is secreted 729073 3.1.1.41 gene TM0077, expression of N-terminally His-tagged wild-type enzyme in Escherichia coli and selenomethionine-labeled enzyme in Escherichia coli methionine auxotrophic strain DL41 730907 3.1.1.41 N-terminally truncated form 651823 3.1.1.41 the most efficient expression plasmid is pCAH431, the expression and purification system has made the industrial production of the enzyme possible 170677