2.7.8.24 cloned into the expression vector pET21b to form the recombinant plasmid pET125, expression in Escherichia coli BL21 662785 2.7.8.24 expressed in Escherichia coli BL21(DE3) pLysS cells 721960 2.7.8.24 expressed in Escherichia coli BL21(DE3).pLysS cells 721725 2.7.8.24 expressed in Escherichia coli BL21(DE3)/pLysS cells 723125 2.7.8.24 expressed in Escherichia coli DH5alpha cells 723115 2.7.8.24 expression in Escherichia coli 662001, 662776 2.7.8.24 from plasmid pGC41 in pGC36 for functional complementation in bacterial mutants lacking endogenous PcsA 691697 2.7.8.24 gene pcsA, DNA and amino acid sequence determination and analysis 738417 2.7.8.24 recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli, recombinant expression of His6-tagged enzyme mutants in Escherichia coli strain C41 (DE3) 738208