2.7.1.190 - 2.7.1.190 expression in Escherichia coli 2.7.1.190 gene aacA-aphD, synthesis and codon optimization of the gene from Staphylococcus aureus for expression in Nicotiana tabacum from the plastid genome, driven by the psbA gene promoter (PpsbA) from Chlamydomonas reinhardtii, generation of transplastomic lines with the three different aac6-aph2 vectors, recombinant expression in tobacco chloroplasts 2.7.1.190 gene aphA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 2.7.1.190 gene aphSR2, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases and localized in one cluster of the Streptomyces rimosus strain ATCC 10970 genome, causing a 2fold increase in resistance to neomycin 2.7.1.190 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3). The catalytic activity of APH(2'')-IVa is independent of the presence of the 6His-tag 2.7.1.190 recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)