2.7.1.151	DNA and amino acid sequence determination and analysis, no functional complementation of yeast mutant strains, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)	640974	
2.7.1.151	DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein	660946	
2.7.1.151	Examinations of plants deficient in IPMK suggest roles in pollen germination, root growth, as well axillary shoot branching.	686905	
2.7.1.151	expressed in Lycopersicon esculentum cv. PKM	706854	
2.7.1.151	expression in bacteria as glutathione S-transferase fusion protein	393221	
2.7.1.151	expression in enzyme-deficient yeast cells, stable overexpression in HEK-293 cells, abrogates the in vivo elevation of 1D-myo-inositol 1,4,5,6-tetrakisphosphate from the Salmonella dublin SopB protein	660926	
2.7.1.151	expression in HEK293 cells as glutathione S-transferase fusion protein	393220	
2.7.1.151	expression in Saccharomyces cerevisiae. A lysine accumulation phenotype of ScIpk2DELTA mutant yeast is rescued by Solanum tuberosum inositol phosphate multikinase	671894	
2.7.1.151	Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria.	689562	
2.7.1.151	expression of HA-tagged enzyme in human HEK293 and in COS-7 cells	663233	
2.7.1.151	expression of wild-type enzyme and deletion mutant in Escherichia coli	662346	
2.7.1.151	gene impk, adeno-associated virus serotype 2-mediated delivery of IPMK into mouse brain, in the striatum of R6/2 Huntington's disease mice	739553	
2.7.1.151	gene impk, recombinant GST-tagged IMPK overexpression in human colon cancer cell line HCT-116 and the human osteosarcoma cell line U2-OS. Overexpression of kinase-deficient IPMK inhibits cell proliferation in etoposide-treated U2OS cells to a similar extent as does wild-type IPMK	739703	
2.7.1.151	gene ipk2 is located on chromosome 10	663252	
2.7.1.151	gene ipmk, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli	761469	
2.7.1.151	gene KCS1, subcloning in Escherichia coli strain XL1-B, overexpression in strain arg82DELTA, expression in strain 12S16cpep4D	662114	
2.7.1.151	genetic map of inositol metabolism, overexpression of isozyme Kcs1 can complement isozyme Ipk2 deficiency	662391	
2.7.1.151	His-tagged enzyme, expression in Escherichia coli	393223	
2.7.1.151	IPMK knockout mice demonstrates the critical roles of IPMK in embryogenesis and central nevous system development. Deletion of IPMK in mice results in early lethality.	686905	
2.7.1.151	isozyme Ipk2alpha, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain	662119	
2.7.1.151	isozyme Ipk2beta, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain	662119	
2.7.1.151	Observed derepression and constitutive elevated levels levels of the transcripts in IPMK mutant yeast but not in ipk1- or ipk6-deficient yeasts confirms the requirement of IPMK in the transcriptional regulation of arginine anabolism and catabolism.	686905	
2.7.1.151	overexpressed in Escherichia coli strain BL21Star	674718	
2.7.1.151	To create an AtIpk2b overexpression construct, the AtIpk2b cDNA is cut from plasmid pmIP3K with XbaI-XhoI and inserted via XbaI-SalI sites into pBinAR-HPT vector behind the cauliflower mosaic virus 35S promoter. The resulting plasmid is first introduced into Agrobacterium tumefaciens by electroporation and then transformed Arabidopsis ecotype C24 by vacuum infiltration. Transgenic plants are selected on MS medium containing 20 mg/L hygromycin. Eleven AtIpk2b overexpression plants, named OX-2, OX-3, OX-5, OX-8, OX-9, OX-15, OX-25, OX-26, OX-33, OX-35, and OX-64, are identified. Compared to the wild type, branching of axillary shoots is more pronounced in the overexpression lines.	689588	
2.7.1.151	While wild-type yeast displays sequential phosphorylation of Ins(1,4,5)P3 to Ins6, mutation of locus termed GSL3 resulted in the loss of Ins(1,4,5)P3-initiated higher isnositol polyphosphate metabolism. IPMK is identified as GSL3 (Ipk2) and confirmed its requirement for mRNA export.	686905