2.7.1.137 - 587104, 640888, 640916, 640936, 640950, 640957, 640959, 640961, 674783 2.7.1.137 C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K 640931 2.7.1.137 CaVPS34 gene under the control of its own promoter is not able to complement the temperature-sensitive growth of Saccharomyces cerevisiae vps34, overexpression of CaVPS34 is sufficient to rescue the temperature-sensitive vps34 phenotype, suggesting a functional conservation in Candida albicans 640934 2.7.1.137 class Ia isozymes, DNA sequence determination and analysis, expression of His-tagged isozymes or P85 and p110 subunits alone in Spodoptera frugiperda Sf9 insect cells utilizing the baculovirus infection system 663296 2.7.1.137 class II phosphoinositide 3-kinase with a C2 domain, cloned from a U937 monocyte cDNA library, expression in mammalian and insect cells 640862 2.7.1.137 class II phosphoinositide 3-kinase, PI 3-kinase C2b, with a C2 domain is cloned from a U937 monocyte cDNA library and expressed in mammalian and insect cells 640862 2.7.1.137 cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin 640880 2.7.1.137 cloning, structural organization, and chromosomal localization of the mouse PI3-kinase p110a gene 640859 2.7.1.137 construction of a tetracycline-inducible expression system by introducing a regulatory plasmid, which constitutively expresses tetracycline-sensitive transactivator, and a response plasmid, containing a tetracycline response element in form of a heptameric repeat of the tetR binding site, under control of a minimal CMV promotor, into Jurkat cells, individual and coexpression of the Myc-tagged enzyme subunits in the Jurkat cell system, determination of expression levels, overview 662576 2.7.1.137 DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain 708996 2.7.1.137 expressed in Escherichia coli 690219 2.7.1.137 expression of GST-tagged PI3K SH3 domain in Escherichia coli strain Bl21 707617 2.7.1.137 expression of the 110000 Da subunit in Sf9 insect cells and in COS-1 cells 640857 2.7.1.137 expression of the catalytic subunit p110alpha of the wild-type and mutant enzymes in COS-1 cells 661328 2.7.1.137 expression of the complete coding sequence as a glutathione S-transferase fusion protein in Sf9 cells 640944 2.7.1.137 expression of the GRP1 domain, comprising residues 263-380, in Escherichia coli 660688 2.7.1.137 expression of the p85 subunit and a related p85beta protein by using baculovirus expression system 640855 2.7.1.137 expression of the wild-type p110 alpha protein in CHO cells 640955 2.7.1.137 expression of wild-type and mutant HA-tagged PI3K isozyme gamma and of subunit p110 in COS-7 cells, stimulation of the protein kinases Erk, JNK, and PKB 660643 2.7.1.137 expression of wild-type VPS34 and PI3-K-related protein MEC1 in a enzyme-knockout mutant strain, functional complementation analysis, overview 661328 2.7.1.137 gene AtVPS34, genetic structure determination, sequence determination and genotyping, overview 710306 2.7.1.137 gene PIK3R2 738948 2.7.1.137 gene RT1035, recombinant expression of wild-type and mutant enzymes in HeLa cells 761792 2.7.1.137 genotyping 708031 2.7.1.137 p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85 alpha into an active p85 alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85 alpha 640857 2.7.1.137 phosphoinositide-3 kinase isotype, p110 gamma 640916 2.7.1.137 PI 3-kinase p170 640921 2.7.1.137 PI3K-C2 gamma gene locus is mapped to the distal region of mouse chromosome 6 in a region of homology with human chromosome 12p 640947 2.7.1.137 PIK3C2G is mapped to chromosome 12 640952 2.7.1.137 stable expression of GFP-tagged Atg14 in NIH3T3 cells, transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Atg14, Beclin 1, and UVRAG in HEK-293T cells 709868 2.7.1.137 transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Beclin 1 in HEK-293T cells 709868 2.7.1.137 transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged UVRAG in HEK-293T cells 709868