2.6.1.51 - 2.6.1.51 binding sites identified by using a luciferase reporter assay with HepG2 cells, DNase I footprinting analysis and gel shift experiments 2.6.1.51 C-terminal SPT mutants constructed and examined in transfected COS-1 cells 2.6.1.51 cDNA cloned and expressed in Escherichia coli DH1 2.6.1.51 cloned and expressed in Escherichia coli DH5 and Escherichia coli ER1458 2.6.1.51 expressed in SF9 cells, using a insect/baculovirus expression system 2.6.1.51 gene AGXT, quantitative real-time reverse transcription-PCR enzyme expression analysis 2.6.1.51 plasmid containing cDNA of human SPT cloned 2.6.1.51 SPT/AGT gene is linked to the CAT gene and transiently expressed in human hepatoma-derived HepG2 cells