2.5.1.93	all point mutants and chimeric enzymes are constitutively expressed in Saccharomyces cerevisiae containing a disrupted copy of the COQ2 gene	
2.5.1.93	expression in Saccharomyces cerevisiae WAT11U strain and in a high yield monoterpene strain (HMT1). AePGT6 shows higher geranyltransferase activity in yeas than AePGT4 and AePGT	
2.5.1.93	functional expression of LePGTs in a yeast COQ2 disruptant	
2.5.1.93	PGT expression analysis	
2.5.1.93	recombinant expression in Escherichia coli coenzyme Q8 biosynthesis-deficient strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. Gene lePGT-1 gene is amplified and cloned into the expression vector, pBAD33, to form pBlePGT, the fine-tuning of the expression of lePGT-1 in the ubiquinone-deficient strains, HW108 and HW109, to control the electron transfer chain is investigated by adding different concentrations of the inducer arabinose at 0-20 mM under fully aerobic conditions	
2.5.1.93	recombinant expression of C-terminally His-tagged LePGT1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method