2.5.1.60 - 2.5.1.60 cloning of the beta subunit of enzyme by means of the yeast two-hybrid system 2.5.1.60 development of a two plasmid expression system in Escherichia coli that achieves large quantities of enzyme 2.5.1.60 expressed in Escherichia coli 2.5.1.60 expressed in Sf9 cells 2.5.1.60 expression in Escherichia coli 2.5.1.60 expression in SF21 cells 2.5.1.60 expression in Sf9 cells with recombinant baculovirus 2.5.1.60 gene RGTA1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA1 subunit in Saccharomyces cerevisiae strain YPH499 2.5.1.60 gene RGTA2, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA2 subunit in Saccharomyces cerevisiae strain YPH499. No detectable RGTA2-c-Myc is present in the pulldown eluate, indicating that RGTA2-c-Myc can only be pulled down in a complex with FLAG-tagged RGTB1/2 2.5.1.60 gene RGTB1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of FLAG-tagged RGTB1 subunit in Saccharomyces cerevisiae strain YPH499 2.5.1.60 gene rgtb1, the gene structure is very similar to that of its paralogue, AtRGTB2, except that the sequence corresponding to the first exon of AtRGTB2 is split into two shorter exons in the AtRGTB1 gene, sequnce comparison and expression analysis 2.5.1.60 gene rgtb2, located on the third chromosome, comprises nine exons, and its structure is very similar to that of its paralogue, AtRGTB1, except that the sequence corresponding to the first exon of AtRGTB2 is split into two shorter exons in the AtRGTB1 gene, sequnce comparison and expression analysis 2.5.1.60 recombinant enzyme produced in baculovirus and Escherichia coli system 2.5.1.60 recombinant enzyme produced in insect cells