2.4.1.90	cloned into pMAL-c4X vector and expressed in Escherichia coli Origami B (DE3) cells as an N-maltose binding protein (MBP)-tagged and C-His6-tagged fusion protein	719274	
2.4.1.90	cloning and sequencing of cDNA	489501	
2.4.1.90	cloning and sequencing of the full-length cDNA	489497	
2.4.1.90	comparison of sequences of enzyme from placenta and HeLa cells	489502	
2.4.1.90	DNA and amino acid sequence determination and analysis, promoter activity analysis	687455	
2.4.1.90	DNA fragments coding the catalytic domain (Cd7) sequence (residues 71-322) and the C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) are cloned into a pET23a vector and expressed in Escherichia coli	719863	
2.4.1.90	enzymatically active soluble N-deglycosylated enzyme form	489554	
2.4.1.90	enzyme fused to protein A is expressed as a soluble form in COS-7 cells	489543	
2.4.1.90	expressed in a silkworm-baculovirus expression vector system	757639	
2.4.1.90	expressed in Escherichia coli BL21(DE3) cells	756991, 758173	
2.4.1.90	expression as GFP-tagged enzyme in HeLa cells	677221	
2.4.1.90	expression in Cos-1 cells	489496	
2.4.1.90	expression in Escherichia coli	489540, 489556	
2.4.1.90	expression in Sf9 cells. Sfbeta4GalT cell, unlike the parental Sf9 cells, can terminally beta1,4-galactosylate gp64 during baculovirus infection	489544	
2.4.1.90	expression of mutant cDNA from a patient with the congenital disorder of glycosylation type IId leads to the synthesis of a truncated, inactive polypeptide, which is localized to the endoplasmic reticulum	489545	
2.4.1.90	expression of short and long enzyme form in CHO-cells	489516	
2.4.1.90	expression of wild-type and mutant enzymes in Escherichia coli	489552	
2.4.1.90	expression of wild-type enzyme and mutant W314A in Escherichia coli in inclusion bodies	659700	
2.4.1.90	expression under the control of the T7 promoter in Escherichia coli BL21	489555	
2.4.1.90	HeLa cells expressing the murine enzyme on their surface spread more rapidly on laminin substrates than do control cells	489539	
2.4.1.90	histidine-tagged 46000 Da protein produced in Escherichia coli	489542	
2.4.1.90	isolation and characterization of the genomic locus	489506	
2.4.1.90	isolation of a cDNA clone that encodes a major portion of galactosyltransferase	489499	
2.4.1.90	molecular cloning and nucleotide sequencing	489507	
2.4.1.90	NmLgtB is cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3) cells as N-terminal His6-tagged fusion protein. Under common expression conditions only a low amount of soluble active enzyme is obtained. The expression level is improved 15fold to 12.5 mg/L culture under osmotic stress condition using LB medium supplemented with 0.2% glucose and a higher concentration of NaCl	719274	
2.4.1.90	quantitative enzyme expression analysis in strains UR6 and AG83, overview	686983	
2.4.1.90	recombinant enzyme is N-glycosylated	489508	
2.4.1.90	transient functional overexpression in 7721 hepatocarcinoma cells	659925