2.4.1.65 - 637638, 639545, 661277, 661433, 661839 2.4.1.65 cloning of chimeric FucTs, expression in Escherichia coli HMS174DE3 cells 662137 2.4.1.65 construction of plasmids encoding soluble forms of the recombinant human FucT-III where the human IL-2 sequence is linked to Ala47 or Val-36 of the FucT-III and expression in stable transfected BHK-21 cell lines 637648 2.4.1.65 expression in BHK-21B cells 637637 2.4.1.65 expression in COS cells 637644 2.4.1.65 expression in COS-1 cell 757939 2.4.1.65 expression in Escherichia coli 680029, 756431 2.4.1.65 expression in HEK 293 cells 660797 2.4.1.65 expression in Saccharomyces cerevisiae, construction of fusion genes PIR1-HA-FUT6 and PIR2-FLAG-FUT6 661803 2.4.1.65 expression in Spodoptera frugiperda (Sf9) insect cells 661313 2.4.1.65 expression in Spodoptera frugiperda SF-9 cells, the secreted activity SFT3 increases until the 6th day of culture when it reaches the value 1.9 mU x 10e-6 cells and 13.4 mg/l, whereas only 5% of activity is retained inside the cells 662484 2.4.1.65 expression of a secreted form of Fuc-TIII, SFT3, in two insect cell lines, Spodoptera frugiperda and Trichoplusia ni using the baculovirus expression system. The enzyme from the Tn cell line has a lower global charge, possibly due to post-translational modifications, such as phosphorylation or sulfation 637643 2.4.1.65 expression of alpha-1,3/4-fucosyltransferase in the gastric pit cell lineage of FVB/N transgenic mice 703893 2.4.1.65 expression of wild-type and mutant enzyme in COS-7 cells 693000 2.4.1.65 full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3 680736 2.4.1.65 plasmid ProtA-FucT-VI, encoding a soluble chimaeric protein consisting of a part of protein A fused to the catalytic domain of FucT-VI, transfected into Cos-7 cells 703056 2.4.1.65 stable expression of FucT IV and IV in CHO cells 658008 2.4.1.65 transient expression of cDNA splice variants FUT10-391, FUT10-419, and FUT10-479, and GFP-tagged-FUT10 cDNA constructs from vector PCR3.1 in COS-7 Cells 704613 2.4.1.65 transient expression of cDNA splice variants FUT11-476 and FUT11-492 from vector PCR3.1 in COS-7 Cells 704613