2.4.1.4	-	
2.4.1.4	cloned into an inducible expression system in Escherichia coli	
2.4.1.4	codon-optimized and expressed in Escherichia coli BL21 (DE3)	
2.4.1.4	DNA library construction and DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli	
2.4.1.4	expression in Escherichia coli	
2.4.1.4	expression in Escherichia coli BL21(DE3)	
2.4.1.4	expression in Eschetrichia coli	
2.4.1.4	expression inn Escherichia coli	
2.4.1.4	expression of GST-tagged ASase in Escherichia coli strain BL21 (DE3)	
2.4.1.4	expression of GST-tagged DgAS	
2.4.1.4	expression of GST-tagged DGAS in Escherichia coli strain BL21	
2.4.1.4	expression of His-tagged NPAS in Escherichia coli strain BL21	
2.4.1.4	expression of wild-type and mutant enzymes in Escherichia coli strain JM109	
2.4.1.4	expression of wild-type and random mutants in Escherichia coli strain JM109	
2.4.1.4	gene acas, locus CP001341, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme	
2.4.1.4	gene ams, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His6-tagged in Escherichia coli strain Rosettta (DE3)	
2.4.1.4	gene ams, recombinant expression as starch-binding domain-fusion protein in Solanum tuberosum plants, cv. Kardal and amf, using the Agrobacterium tumefaciens transformation method	
2.4.1.4	gene amsA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLyS	
2.4.1.4	gene dgas, DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain JM109	
2.4.1.4	gene dgas, expression of GST-tagged DGAS in Escherichia coli	
2.4.1.4	gene dgas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	
2.4.1.4	gene drpas, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of the His-tagged enzyme in Escherichia coli	
2.4.1.4	gene mfas, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	
2.4.1.4	gene npas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	
2.4.1.4	heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum	
2.4.1.4	His6-tagged enzyme expression in Escherichia coli strain MC1061 using a constitutive expression system	
2.4.1.4	overexpressed in Escherchia coli	
2.4.1.4	potential use for the synthesis or the modification of polysaccharides is limited by its low catalytic efficiency on sucrose alone, its low stability, and its side reactions resulting in sucrose isomer formation. Development of a zero background expression cloning strategy for the generation of large variant libraries, a selection mechanism to discard inactive variants, and a screening method for identification of interesting clones	
2.4.1.4	recombinant expression in Escherichia coli strain BL21	
2.4.1.4	recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109	
2.4.1.4	recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109