2.4.1.236 1,2RhaT cloning and DNA and amino acid sequence determination and analysis, phylogenetic analysis, two native frameshift mutations render the enzyme from orange catalytically inactive 2.4.1.236 cloned into the binary vector PH7WG2D under the control of the CAMV-35S promoter and translationally fused to the green fluorescent protein reporter gene. The recombinant vector is then transferred into Agrobacterium tumefaciens 2.4.1.236 DNA and amino acid sequence determiation and analysis, phylogenetic analysis, recombinant expression of C-terminally His6-Myc-tagged enzyme in Nicotiana tabacum BY-2 cells 2.4.1.236 gene 1,2-Rhat, DN and amino acid sequence determination and analysis of in varieties Anliu and Honganliu sweet orange, sequence comparisons 2.4.1.236 phylogenetic analysis, 1,2RhaT expressionin BY-2 cells