2.4.1.155 a recombinant vector MGAT5-pCXNneo is constructed for transfection of WiDr cells 694174 2.4.1.155 fusion with signal peptide from baculoviral protein to permit secretion into medium, BVVdelta73 and BVV delta188 638558 2.4.1.155 gene expression profiling of cells is conducted by DNA microarray analysis 759884 2.4.1.155 gene MGAT5, overexpression in CHO cells and Lec4 cells from Cricetulus griseus 736218 2.4.1.155 gene MGAT5, quantitative real-time PCR enzyme expression analysis 721738, 735590, 759648 2.4.1.155 gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144 735724 2.4.1.155 gene MGAT5, recombinant expression of enzyme Gnt-V in CNE-1 and CNE-2 cells using the lentiviral transfection method 759306 2.4.1.155 gene MGAT5, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat5 HEK293 cells display a 2fold increase in tri-antennary and a 2fold increase in tetraantennary N-glycans 736200 2.4.1.155 Huh7 and HepG2 cells are transiently transfected by both Mgat5 expression vector plasmid and empty vector plasmid as a control 736192 2.4.1.155 into the pcDNA3 vector for transfection of rat pheochromocytoma cells, PC12 691708 2.4.1.155 into the pGEM-Teasy vector and subcloned into the pcDNA3 expression vector 690452 2.4.1.155 MKN45 cells are transfected with GnT-III, GnT-V, or both, respectively 674676 2.4.1.155 stable overexpression of N-acetylglucosaminyltransferase V (GnT-V) in breast cancer MCF-10A cell line 758997 2.4.1.155 transfection in HEK-293 cells 703908 2.4.1.155 transfection into 7721 cells 704793 2.4.1.155 wild type enzyme and several N- and C-terminal deletions, fusion to protein A from Staphylococcus aurus 638554