2.4.1.146 cloning of a sequence fragment encoding the soluble catalytic domain, expression as His-tagged protein in CHO cells 2.4.1.146 gene B3GNT3, recombinant expression in CHO-K1 cells. Glycosylation-related genes in CHO-K1 cells are present, but strictly suppressed and regulated. CHO cells are transiently co-transfected with plasmids encoding extended C1 beta3GnT3, C2 beta6GnT1, or C3 beta3GnT6 with or without ST6Gal I or CHST4, respectively, and resulting O-glycan and core chain spectrum, overview 2.4.1.146 isolation and cloning of full length DNA via EST and expression in CHO cells