2.3.1.74 - 486402, 487453, 487476, 487479, 487482, 487483, 487484, 673859, 673860, 689725 2.3.1.74 an octaploid (Fragaria x ananassa cv. Calypso) genotype of strawberry is transformed with an antisense chalcone synthase (CHS) gene construct using a ripening related CHS cDNA from Fragaria x ananassa cv. Elsanta under the control of the constitutive CaMV 35S promoter via Agrobacterium tumefaciens. Out of 25 transgenic lines, nine lines showed a reduction in CHS mRNA accumulation of more than 50% as compared to the untransformed cv. Calypso control 674119 2.3.1.74 expressed in Arabidopsis thaliana mesophyll protoplasts 755796 2.3.1.74 expressed in Escherichia coli 676378 2.3.1.74 expressed in Escherichia coli BL21 (DE3) pLysS cells 757913 2.3.1.74 expressed in Escherichia coli BL21 cells 757489, 757756 2.3.1.74 expressed in Escherichia coli BL21(DE3) cells 757784, 758151 2.3.1.74 expressed in Escherichia coli BL21-Rosetta (DE3) cells 756731 2.3.1.74 expressed in Escherichia coli DH5alpha cells 756166, 757938 2.3.1.74 expressed in Escherichia coli Rosetta-gami (DE3) and BL21-Rosetta (DE3) cells 757487 2.3.1.74 expressed in Nicotiana tabacum 756859 2.3.1.74 expressed in Nicotiana tabacum leaves 757970 2.3.1.74 expression in Escherichia coi. 672501 2.3.1.74 expression in Escherichia coli 684699, 719618, 757991 2.3.1.74 expression in Escherichia coli JM109 701213 2.3.1.74 expression in Escherichia coli, site directed mutagenesis, sequence alignment with EC 2.3.1.95 487455 2.3.1.74 expression of the coding sequence of Pchs in Nicotiana tabacum can lead to darker flower limbs than seen in controls, and some transgenic plants exhibited abnormality in growth of pollen tubes 676562 2.3.1.74 fusion protein with glutathione S-transferase 3737 2.3.1.74 GCHS2 and GCHS26 with different enzymatic and structural properties 487475 2.3.1.74 gene CHS and mutant gene CHS-wf, DNA and amino acid sequence determination and analysis, expression analysis by semi-quantitative RT-PCR 705626 2.3.1.74 gene CHS, DNA and amino acid sequence determination and analysis, expression analysis 702519 2.3.1.74 gene CHS, realtime PCR expression analysis 706186 2.3.1.74 gene CHS-A, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree 705645 2.3.1.74 gene CHS-B, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree 705645 2.3.1.74 gene CHS-C, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree 705645 2.3.1.74 gene CHSL1, DNA and amino acid sequence determination and analysis, pattern of expression, overview 706185 2.3.1.74 gene CsCHS-bo, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, cloning and expression in Escherichia coli strain DH5alpha 705629 2.3.1.74 hairy roots, transformed with the soybean chalcone synthase (CHS6) or isoflavone synthase (IFS2) genes, with dramatically decreased capacity to synthesize isoflavones are produced to determine what effects these changes would have on susceptibility to a fungal pathogen 676727 2.3.1.74 infiltration of Nicotiana benthamiana leaves with chs_H1 promoter/GUS chimeras leads to a 24.8-fold increase of the GUS activity when coinfiltrated with the pap1 gene. Coinfiltration of the native chs_H1 gene with pap1 leads to an increased accumulation of chs_H1 mRNA. Transgenic lines of Petunia hybrida expressing the pap1 gene showed unusual patterns of UV-A-inducible pigmentation and anthocyanin accumulation in parenchymatic and medulla cells. Infiltration of transgenic leaves of Petunia hybrida with chs_H1 and pap1 genes arranged as a tandem led to quick pigmentation within 12 h after UV-A irradiation 674125 2.3.1.74 introduction of the phenylpropanoid pathway with the genes for phenylalanine ammonia lyase (PAL) from Rhodosporidium toruloides, 4-coumarate:coenzyme A (CoA) ligase (4CL) from Arabidopsis thaliana, and chalcone synthase (CHS) from Hypericum androsaemum into two Saccharomyces cerevisiae strains, namely, AH22 and a pad1 knockout mutant. Each gene is cloned and inserted into an expression vector under the control of a separate individual GAL10 promoter 660760 2.3.1.74 isoforms 487480 2.3.1.74 isolation and characterization of cDNA sequences encoding yellow lupin chalcone synthase. Chalcone synthase is encoded by at least two genes. The two sequences may have evolved by gene duplication 753216 2.3.1.74 overexpressed in Escherichia coli 757487 2.3.1.74 overexpression in Escherichia coli as glutathione-S-transferase fusion protein, wild-type and mutant enzymes: L263M, F265Y, G256A, S338G, L263M/F265Y, G256A/S338G, L263M/S338G, F265Y/S338G, L263M/F265Y/S338G, G256A/L263M/F265Y, G256A/L263M/F265Y/S338G 660170 2.3.1.74 recombinant Escherichia coli cells containing four genes for a phenylalanine ammonia-lyase, cinnamate/coumarate:CoA ligase, chalcone synthase, and chalcone isomerase, in addition to the acetyl-CoA carboxylase, have been established for efficient production of (2S)-naringenin from tyrosine and (2S)-pinocembrin from phenylalanine. Further introduction of the flavone synthase I gene from Petroselinum crispum under the control of the T7 promoter and the synthetic ribosome-binding sequence in pACYCDuet-1 causes the Escherichia coli cells to produce flavones: apigenin (13 mg/l) from tyrosine and chrysin (9.4 mg/l) from phenylalanine 671524 2.3.1.74 wild type and mutants 487481 2.3.1.74 wild type enzyme and HvCHS2 with different substrate requirements 487474