2.3.1.286 - 730287, 730706, 730840, 730947, 750907 2.3.1.286 a gene encoding the C-terminally His-tagged SIRT1 catalytic domain is expressed in SF9 cells 730166 2.3.1.286 expressed in Escherichia coli 751547 2.3.1.286 expressed in Escherichia coli BL21 cells 749470 2.3.1.286 expressed in Escherichia coli BL21(DE3) and B834 (DE3) cells 751318 2.3.1.286 expressed in Escherichia coli BL21(DE3) cells 739668, 751017, 752068 2.3.1.286 expressed in Escherichia coli BL21(DE3) CodonPlus RIPL cells 737389 2.3.1.286 expressed in Escherichia coli Codon-plus RIPL cells 749886 2.3.1.286 expressed in Escherichia coli XL1Blue cells 750253 2.3.1.286 expressed in Mycobacterium smegmatis mc2155 cells 749818 2.3.1.286 expressed in Sf9 insect cells 752023 2.3.1.286 expression in Escherichia coli 718508, 730205, 730713 2.3.1.286 gene patZ, sequence comparisons and phylogenetic analysis, recombinant enzyme overexpression in Escherichia coli strain BW25113 757557 2.3.1.286 gene phnO, sequence comparisons and phylogenetic analysis, recombinant enzyme overexpression of wild-type and mutant enzymes in Escherichia coli strain BW25113 757557 2.3.1.286 gene yiaC, sequence comparisons and phylogenetic analysis, recombinant enzyme overexpression of wild-type and mutant enzymes in Escherichia coli strain BW25113 757557 2.3.1.286 gene yjaB, sequence comparisons and phylogenetic analysis, recombinant enzyme overexpression of wild-type and mutant enzymes in Escherichia coli strain BW25113 757557 2.3.1.286 recombinant human SIRT2 (43-356) is expressed as a His-Sumo fusion protein in Escherichia coli 729603 2.3.1.286 SIRT1-FL (full-length human SIRT1) is cloned in the pECE vector (clone 1791) and N-terminally truncated SIRT1 (SIRT1-NT with residues 7–83 deleted) cloned in the pCDNA3.1 vector. To express the FLAGtagged hSIRT1 (human SIRT1) proteins, the hSIRT1 constructs are transfected into HEK (human embryonic kidney)-293T cells 729164