2.3.1.158 DNA sequence determination and analysis, functional overexpression in Arabidopsis thaliana roots and leaves 660241 2.3.1.158 expressed in Pichia pastoris 755830 2.3.1.158 expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter 685341 2.3.1.158 expression in Saccharomyces cerevisiae 736422 2.3.1.158 expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves 756132 2.3.1.158 expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves. CsPDAT1-A shows a higher preference for alpha-linolenic acid (18:3 omega-3) 756132 2.3.1.158 gene encoding PDAT, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of full-length enzyme and of truncated PDAT lacking the transmembrane domain in Pichia pastoris 720694 2.3.1.158 gene LRO1, DNA and amino acid sequence determination and analysis 757544 2.3.1.158 gene MiPDAT, DNA and amino acid sequence determination and analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthamiana leaves via transfection method with Agrobacterium tumefaciens, subcloning in Escherichia coli strain DH5alpha, recombinant expression of PDAT in TAG-deficient Saccharomyces cerevisiae mutant strain H1246, complementation and lipid analysis, overview 758363 2.3.1.158 gene pdat or YALI0E16797g, DNA and amino acid sequence determination and analysis. In vivo expression of DGAT1, DGAT2 and PDAT under the heterologous Yarrowia lipolytica YAT promoter results in 598%, 702% and 278% increases in total fatty acid % dry cell weight over the empty vector control 721119 2.3.1.158 gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, lipid analysis, overview 758030 2.3.1.158 gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, SiPDAT1-expressing mutants have higher polyunsaturated (C18:1; C18:2) fatty acid content, lipid analysis, overview 758030 2.3.1.158 infiltrated into the lower epidermal cells of tobacco leaves via Agrobacterium tumefaciens GV3101 758363 2.3.1.158 isozyme PDAT2 and PDAT1A, cloning from cDNA library, expression in Arabidopsis thaliana, coexpression of PDAT1A with Ricinus communis hydroxylated fatty acid hydroxylase, RcFAH, in Arabidopsis thaliana causes a reduction of fatty acids by 73% compared to wild-type plants, isozyme PDAT2 is also functional but less active than isozyme PDAT1A, method optimization, phenotypes, overview 720737 2.3.1.158 overexpression in Arabidopsis thaliana increases alpha-linolenic acis content in seed oil 757544 2.3.1.158 overexpression in Arabidopsis thaliana increases hydroxy fatty acid in seed oil 757544 2.3.1.158 the gene encoding the enzyme is YNR008w, overexpression of the enzyme-encoding gene increases triacylglycerol content in yeast cells 393082