2.3.1.133 - 737024 2.3.1.133 an antisense construct is generated for down-regulation of HCT 689416 2.3.1.133 expressed in Escherichia coli 706306 2.3.1.133 expression in Escherichia coli 486285, 736977, 757945, 758066 2.3.1.133 expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3) 720173 2.3.1.133 gene cbhct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged HST in Escherichia coli strain BL21(DE3)pLysS 720787 2.3.1.133 gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3) 720638 2.3.1.133 genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3) 720173 2.3.1.133 overexpression of N-terminally His6-tagged HCT in Escherichia coli strain BL21 Star(DE3)pLysS using a commercially available synthetic gene encoding HCT with codons optimized for Escherichia coli expression 718519 2.3.1.133 recombinantly expressed in Escherichia coli 702918 2.3.1.133 the amplified PCR fragments of the putative HCT cDNA clone are sequenced and cloned into a derivative of pAHC25, the resulting plasmid containing the HCT RNAi construct is named pHF5 689750