2.3.1.12 - 348961, 348982, 393963, 486166, 486169, 486171, 486179, 486224, 486227, 486228, 486229, 486233, 486236, 486238, 486240 2.3.1.12 a recombinant fragment hL2S (containing the second lipoyl domain (L2), second hinge region, E1-binding domain (S) and third hinge region of hE2, residues 128–330) is overexpressed in Escherichia coli 686772 2.3.1.12 catalytic domain, residues 173-427 486225 2.3.1.12 expressed in Escherichia coli 672273, 690088 2.3.1.12 expressed in Escherichia coli AG1 cells 736459 2.3.1.12 expressed in Escherichia coli BL21(DE3) cells 725521, 736448 2.3.1.12 expression in Escherichia coli 720722 2.3.1.12 expression of subgene for lipoyl domain in Escherichia coli 486167 2.3.1.12 expression of the enzymes' L2 domain as GST-fusion protein 662151 2.3.1.12 functional overexpression of the enzyme E2 with a single hybrid lipoyl domain per subunit, i.e. 1-lip E2, in strain JRG1342 and BL21(DE3), overexpression of the isolated hybrid lipoyl domain in strain JM 101 663289 2.3.1.12 gene pdhC, organized in the pdhCD operon, physical map, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli 661817 2.3.1.12 inner lipoyl domain (L2) of dihydrolipoyl acetyltransferase is expressed as a GST fusion protein 702229 2.3.1.12 knock-out mutants 486239 2.3.1.12 overexpression of truncated thrombin-cleavable enzyme in Escherichia coli strain BL21(DE3) 661632 2.3.1.12 the inner lipoyl-bearing domain of dihydrolipoamide transacetylase is expressed as a GST fusion protein in Escherichia coli 704423 2.3.1.12 wild-type and deletion mutants, expression in Escherichia coli 486170 2.3.1.12 wild-type and mutants 486237