2.3.1.107	enzyme is expressed as recombinant His-tagged protein in Escherichia coli	486112	
2.3.1.107	expression in Escherichia coli	486114	
2.3.1.107	gene Dat, DNA sequence determination and analysis	660317	
2.3.1.107	expressed in Escherichia coli and Catharanthus roseus	689417	
2.3.1.107	transformation to Agrobacterium tumefaciens	706200	
2.3.1.107	transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker	712866	
2.3.1.107	the CrDAT transgene is transferred into Catharanthus roseus by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The Catharanthus roseus DAT gene is introduced into Catharanthus roseus plants and it ias confirmed that CrDAT is successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein	765187