2.1.1.86 all of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea 2.1.1.86 expressed in Escherichia coli BL21(DE3) cells 2.1.1.86 expressed in Escherichia coli Rosetta (DE3) cells