2.1.1.67	-	
2.1.1.67	a 8.7 kb fragment encompassing part of exon III through a portion of intron 6 is subcloned into the pZERO-2 vector	
2.1.1.67	baculovirus expressed	
2.1.1.67	cDNA cloned and heterologous expressed in rabbit reticulocytes and wheat germ lysate	
2.1.1.67	cDNA cloning, heterologous expression in yeast	
2.1.1.67	enhanced green fluorescent protein-tagged enzyme is expressed in Escherichia coli, Jurkat, Hep-G2, and HEK-293 cells	
2.1.1.67	expressed in COS-1 cells	
2.1.1.67	expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells	
2.1.1.67	expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	
2.1.1.67	for cloning of the TPMT fragment the TOPO TA cloning kit is used	
2.1.1.67	into the pCR2.1-TOPO vector, used as a template to generate point mutations, the variants are sequenced and cloned into the mammalian expression vector pCMV6-XL5, into the pENTR/D-TOPO vector and subsequently into the pcDNA-DEST40 vector	
2.1.1.67	into the yeast expression vector pYeDP60 for transformation of Saccharomyces cerevisiae cells	
2.1.1.67	retroviral gene transfer	
2.1.1.67	the full-length coding region is amplified by PCR from a eukaryotic expression plasmid and cloned into the pET-28a vector for expression in Escherichia coli BL21DE3 cells	
2.1.1.67	to assess the functional effect of the trinucleotide repeat variants in the promoter, the wild-type and variant TPMT promoters are amplified and cloned into the promoter-less pGL3-Basic vector