2.1.1.56 - 734586, 735240 2.1.1.56 2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli 485455, 485463 2.1.1.56 as a His6-Smt3 fusion protein in Escherichia coli BL21(DE3) CodonPlus RIL using a pET-based pSmt3-TOPO vector 674701 2.1.1.56 coexpression of genes D1 and D12 from 1 plasmid 485463 2.1.1.56 complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 120-476 and being located at the nucleus, no complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 144-476 and being loacetd mainly in the cytoplasm, truncated enzymes are expresss in fusion with GFP 659939 2.1.1.56 D1 fragment, amino acids 498 to 844 485467, 485472 2.1.1.56 D1 fragment, amino acids 520-844 485468 2.1.1.56 expressed in Escherichia coli Rosetta (DE3) cells 758535 2.1.1.56 expressed in Escherichia coli T7 cells 757853 2.1.1.56 expressed in HEK-293 cells and Escherichia coli 757840 2.1.1.56 expressed in Saccharomyces cerevisiae strains YBS40, YBS2, and YBS20 706535 2.1.1.56 expression in Escherichia coli 485466 2.1.1.56 expression in Escherichia coli, His-tagged 485470 2.1.1.56 expression in Escherichia coli, His-tagged, amino acid sequence analysis 485470 2.1.1.56 expression in Saccharomyces cerevisiae, His-tagged, amino acid sequence analysis 485469 2.1.1.56 expression of 2 different variants, each with and without His-tag, in Escherichia coli, amino acid sequence analysis 485469 2.1.1.56 expression of D1 fragment mutants in Escherichia coli 485472 2.1.1.56 expression of full length and 2 truncated genes in Saccharomyces cerevisiae and Escherichia coli, location of methyltransferase activity 485473 2.1.1.56 expression of full length enzyme and truncated enzyme in yeast and in HeLa cells, His-tagged, as GFP-fusion protein, or cMyc-tagged 485471 2.1.1.56 expression of N-terminally His6-tagged enzyme, residues 1-298, as Smt3-fusion protein in Escherichia coli strain BL21(DE3) or strain B834DE3, expression of wild-type and mutant alleles under control of a yeast promotor in a Saccharomyces cerevisiae abd1DELTA null mutant strain 659921 2.1.1.56 expression of VP4 protein in Spodoptera frugiperda cells 485465 2.1.1.56 His6-tagged proteins are expressed in Escherichia coli BL21(DE3) cells and in Saccharomyces cerevisiae strain YBS40 706721 2.1.1.56 in vitro transcription and translation in presence of liposomes, enzyme is attached to the liposomes surface 485466 2.1.1.56 individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli 485467, 485468, 485472 2.1.1.56 inserted into vector pET16b and transformed into Escherichia coli BL21(DE3), yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1 674439 2.1.1.56 into vector pDest14 and transformed into Escherichia coli Rosetta(DE3) cells 676231 2.1.1.56 TbCmt1 inserted into an inducible T7 RNA polymerase-based pET vector and introduced into Escherichia coli BL21(DE3)Gold 677098 2.1.1.56 the MTase domain, representing the N-terminal 272 amino acids of DENV-4 NS5 is cloned and purified 721100 2.1.1.56 vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 485455, 485456, 485463 2.1.1.56 wild-type or mutant vD12, into the pRS413T vector, expressed in Saccharomyces cerevisiae strain YBS40 677246 2.1.1.56 yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1, the pET16-Ecm1 plasmids introduced into Escherichia coli BL21(DE3) 674701