2.1.1.179	-	710880, 710881, 712741	
2.1.1.179	cloned in Streptomyces lividans. The sgm gene is expressed in Micromonospora melanosporea, where its own promoter is active, and also in Escherichia coli under the control of the lacZ promoter	704248	
2.1.1.179	cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression	705988	
2.1.1.179	expressed in Escherichia coli BL21(DE3) cells	733120	
2.1.1.179	expression in Escherichia coli	702415, 704280	
2.1.1.179	expression in Escherichia coli, Bacillus subtilis or Staphylococcus aureus	712038	
2.1.1.179	gene fmrO, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene grmA, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene grmB, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene kmr, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene nbrB, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene rmtA, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene rmtB, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene rmtC, expression of wild-type enzyme in Escherichi coli Top10 cells	718596	
2.1.1.179	gene rmtC, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene rmtD2, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene rmtF encoded on a non-self-transferable plasmid pIP849, cotranscribed with the upstream aac(6')-Ib gene, DNA and amino acid sequence determination, analysis, and comparisons, overview. Genetic structure and phylogenetic tree	718603	
2.1.1.179	gene sgm, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	gene smr1, phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit	706724	
2.1.1.179	phylogenetic analysis, sequence comparisons	719353	
2.1.1.179	sgm is cloned into a yeast expression vector to test whether the prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to the aminoglycoside antibiotic G-418. Sgm does not provide resistant phenotypes to yeast cells. Despite all similarities in the antibiotic binding site, methylation by Sgm does not occur in yeast, suggesting that the recognition site for these methylases could be different in 30S and 40S subunits	706701