1.8.98.2 - 698953, 724192 1.8.98.2 DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type Srx and mutants in Escherichia coli strain BL21(DE3) 712607 1.8.98.2 expressed in Escherichia coli BL21(DE3) cells 742601 1.8.98.2 expressed in HEK293 cells and Escherichia coli 668161 1.8.98.2 expressed in Saccharomyces cerevisiae 668202 1.8.98.2 expression in Escherichia coli 659421, 725015 1.8.98.2 for expression in Escherichia coli BL21DE3 cells 698928 1.8.98.2 gene SRX, overexpression of recombinant His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) 764076 1.8.98.2 into the vector pcDNA3 700520 1.8.98.2 into the vector pET14b for expression in Escherichia coli BL21 cells 695553 1.8.98.2 the 5' flanking region of the human Srx1 promoter region, -3664 - +19, is ligated into the vector pCR2.1 698052 1.8.98.2 the vectors pcDNA3 and pFLAG-CMV2 are used, a leader sequence is cloned into the N-terminus coding region of Srx 698993 1.8.98.2 transient expression of an AtSrx-GFP fusion in Nicotiana tabacum leaves 670564 1.8.98.2 transient recombinant expression of the FLAG-tagged enzyme in different cell types, including HEK293T, H226, and A549 cells, recombinant expression of FLAG-tagged Srx mutant C99A mutant in HEK-293T cells. The Srx-protein complex is pulled down by anti-FLAG immunoprecipitation (IP), and protein identities are determined by mass spectrometry. Among the handful of proteins identified, Prx4 is the most abundant protein, and TXNDC5 is the second most abundant protein that is specifically pulled down by anti-FLAG IP in all cell types. c-Myc-tagged TXNDC5 or its cysteine-specific mutants are expressed in HEK-293T-FLAG-Srx cells 765061