1.7.1.4 expression in Escherichia coli 1.7.1.4 expression in Escherichia coli as lacZ transcriptional fusion 1.7.1.4 gene nir1, DNA and amino acid sequence determination and analysis, gene nir1 is part of the nitrate assimilation gene cluster together with genes encoding a high-affinity nitrate transporter and a nitrate reductase, the intergenic region could also act as bidirectional promotor, organisation of coding sequences