1.5.1.21 DNA sequence determination and analysis, overexpression in Escherichia coli 1.5.1.21 gene lhpD, DNA and amino acid sequence determination and analysis, phylogenetic tree, genetic organization of genes encoding enzyme involved in the trans-3-hydroxy-L-proline pathway and networks with D-lysine and D-proline, overview. Recombinant expression of His6-tagged enzyme in Escherichia coli