1.4.3.11 expressed in Escherichia coli BL21(DE3) cells 742177, 742179, 742944 1.4.3.11 expressed in Pichia pastoris GS115 cells 743742 1.4.3.11 expression in Escherichia coli 763989 1.4.3.11 expression in Escherichia coli strain JM109 711975 1.4.3.11 expression in Escherichia coli. The recombinant enzyme exhibits low thermostability compared to the enzyme isolated from Streptomyces sp. X-119-6 and is an aggregated form. Proteolysis of the recombinant enzyme with metalloendopeptidase from Streptomyces griseus improves the catalytic efficiency at various pH-values and stabilizes the structure of the enzyme 655925 1.4.3.11 mutant enzymes are expressed in Escherichia coli JM109 cells 724186 1.4.3.11 overexpression in Escherichia coli JM109 677135 1.4.3.11 successfully expressed by using pHBM905BDM in Pichia pastoris 764356