1.3.7.5 into vector pET-21a and expressed in Escherichia coli C41(DE3) 676847 1.3.7.5 PcyA expression in Escherichia coli strain BL21(DE3) 712008 1.3.7.5 recombinant expression of GST-tagged PcyA in Escherichia coli 724287 1.3.7.5 recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview 724317 1.3.7.5 subcloned into pKT210, co-expression with heme oxygenase in Escherichia coli cells 673636 1.3.7.5 wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha 674669