1.3.2.3 - 1.3.2.3 cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview 1.3.2.3 expressed in Escherichia coli BL21(DE3) cells 1.3.2.3 expressed in tobacco plants by Agrobacterium-mediated transformation under CaMV 35S constitutive promoter 1.3.2.3 expression in Escherichia coli 1.3.2.3 expression in Saccharomyces cerevisiae 1.3.2.3 for expression in Escherichia coli BL21DE3 cells 1.3.2.3 gene GDH-A1, from the nulli-tetrasomic lines of CS, chromosome 5A, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings 1.3.2.3 gene GDH-B1, from the nulli-tetrasomic lines of CS, chromosome 5B, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings 1.3.2.3 gene GDH-D1, from the nulli-tetrasomic lines of CS, chromosome 5D, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings 1.3.2.3 gene GLDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant 19fold overexpression in Lactuca sativa under the control of the pea plastocyanin promoter and via Agrobacterium tumefcáciens strain LBA4404 mediated transformation, semi-quantitative and quantitative real-time RT-PCR enzyme expression analysis. Transformation experiments in lettuce with gene under the control of the CaMV 35S promoter fails 1.3.2.3 gene GLDH, quantitative real-time RT-PCR enzyme expression analysis 1.3.2.3 gene GLDH1, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis 1.3.2.3 gene L-GalLDH1, quantitative real-time PCR isozyme expression analysis 1.3.2.3 gene L-GalLDH2, quantitative real-time PCR isozyme expression analysis 1.3.2.3 GLDH sense and antisense are transformed into rice callus by Agrobacterium-mediated infection (strain EHA105) 1.3.2.3 into a pET-His6 vector for expression in Escherichia coli BL21DE3 cells 1.3.2.3 into pMAT037 and transformed into the EHA101 strain of Agrobacterium tumefaciens 1.3.2.3 overexpressed in lettuce Agrobacterium-mediated transformation