1.3.1.33 - 393853, 393858, 393859, 656905 1.3.1.33 a genomic library is constructed, the vector pZERO is used 700718 1.3.1.33 barley POR expressed in Escherichia coli as a beta-galactosidase fusion protein 393853 1.3.1.33 cDNA library constructed in pBR322, full-length cDNA cloned, cDNA inserts subcloned in the M13 phage and expressed in Escherichia coli 393851 1.3.1.33 cDNAs and/or gens encoding POR, also referred to as LPCR or PCR in some studies isolated 393853 1.3.1.33 cloned and overproduced in Escherichia coli with a hexahistidine tag at the N-terminus 393863 1.3.1.33 cloning of the gene encoding protochlorophyllide reductase 393865 1.3.1.33 cloning of the nuclear gene 393864, 393865 1.3.1.33 expressed in Escherichia coli 687626, 687767, 699004 1.3.1.33 expressed in Escherichia coli BL21(DE3) cells 740894 1.3.1.33 expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells 687688 1.3.1.33 expressed in Escherichia coli BL21(DE3) pLysS cells 740086 1.3.1.33 expressed in Escherichia coli strain SG13009 689784 1.3.1.33 expressed in Rhodobacter capsulatus SB1003, JDA, JDB and ZY5 393854 1.3.1.33 expression in Escherichia coli BL21 654419 1.3.1.33 expression in Escherichia coli cells 696223, 698913, 698961, 699004, 700758 1.3.1.33 expression in Escherichia coli with a Strep-tag 763334 1.3.1.33 expression in Rhodobacter capsulatus mutant deficient in Bchl biosynthesis. The NADPH:protochlorophyllide oxidoreductase is integrated in the porphyrin biosynthesis network and its activity leads to the formation of photosynthetic chlorophyll proteins 656904 1.3.1.33 for sequencing 697680 1.3.1.33 full-length cDNA, coding for PCR 393851, 393853, 393861, 393865 1.3.1.33 into the vector pQE30 700943 1.3.1.33 mature pea POR cloned into the pAlter-1 mutagenesis vector, expressed in Escherichia coli JM109 393858 1.3.1.33 optimized and expressed in Escherichia coli 763650 1.3.1.33 overexpressed as a fusion with maltose-binding protein in Escherichia coli using expression plasmid pKK233-2 393856, 393857 1.3.1.33 overexpressed in Escherichia coli as a fusion protein with the maltose-binding protein 393860 1.3.1.33 overexpression in Escherichia coli 711484 1.3.1.33 overexpression in Escherichia coli as a fusion protein with maltose-binding protein 656908 1.3.1.33 overexpression in Escherichia coli as a His6-tagged protein 656908 1.3.1.33 POR C cloned and expressed in Escherichia coli 393865 1.3.1.33 subcloned into the expression vector pRSETA and recombinantly expressed in Escherichia coli SoluBL21 762601 1.3.1.33 the coding regions of LPOR (amino acids 35-397) is amplified from pBDCrPCYA1-FDBR vector, cloned into pGADT7 vector, and introduced into Y187 yeast cells, recombinant expression of MBP-tagged enzyme LPOR. Yeast two-hybrid and pull down assay analyses of protein-protein interaction 763107 1.3.1.33 yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells 700758 1.3.1.33 yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells, for the construction of transgenic plants pDONR/ZeO and pGWB2 vectors are used 700758