1.21.99.3	-	
1.21.99.3	DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells	
1.21.99.3	expressed in Escherichia coli Rosetta2 cells	
1.21.99.3	expressed in Xenopus laevis oocytes	
1.21.99.3	expression in oocytes from Xenopus laevis	
1.21.99.3	expression of RNAi to knock down D3 in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes	
1.21.99.3	expression of RNAi to knock down type 3 deiodinase in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes	
1.21.99.3	isozyme type III, expression of wild-type and active site mutants in COS cells, 50fold higher expression level for the mutants than for the wild-type enzyme	
1.21.99.3	isozyme type III, via RT-PCR from cerebellum mRNA, expression in bacteria	
1.21.99.3	screening of neonatal skin cDNA library, DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, functional translation in Xenopus laevis oocytes, catalytis properties of recombinant and native enzyme are identical	
1.21.99.3	the enzyme is a selenoenzyme encoded by the DIO3 gene, localized on chromosome 14q32, in humans. The DIO3 genomic structure contains a single exon and, at the 3'-UTR, a specific RNA structure, named SECIS (selenocysteine insertion element), that is crucial for the insertion of the selenocysteine residue and for maximal enzymatic catalytic efficiency. The DIO3 gene is imprinted, with preferential expression of the paternal allele. It belongs to a cluster of imprinted regions, at the DLK1-DIO3 locus	
1.21.99.3	transfection in HEK 293 cells