1.2.1.70 AtHemA1 overexpression in Nicotiana tabacum cv Samsun NN using Agrobacterium tumefaciens and Arabidopsis thaliana by transformation 1.2.1.70 DNA and amino acid sequence determination and analysis, phylogenetic tree 1.2.1.70 expressed in Escherichia coli BL21 cells 1.2.1.70 expression as His-tag fusion protein in Escherichia coli 1.2.1.70 expression in Escherichia coli 1.2.1.70 expression in Escherichia coli as a fusion protein with glutathione S-transferase 1.2.1.70 expression in Escherichia coli BL21 1.2.1.70 expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme 1.2.1.70 gene HEMA1 1.2.1.70 gene HEMA1, recombinant co-overexpression of N-terminally His6-tagged FLUTPR and GluTRDD in Escherichia coli strain BL21(DE3) 1.2.1.70 hemA ligated into pUC19 vector with a Lys insert between Thr-2 and Leu-3 at N terminus, overexpression in Escherichia coli 1.2.1.70 His(6)-GluTR overexpressed in Escherichia coli BL21 (DE3) 1.2.1.70 N-terminal His6-tagged GluTR expressed in Escherichia coli 1.2.1.70 overexpression in Escherichia coli 1.2.1.70 recombinant enzyme expression in Rhodobacter sphaeroides strain DSM 5864, which synthesizes 5-aminolevulinic acid through the C-4 pathway and also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. Expression of the glutamyl-tRNA reductase gene from Rhodospirillum rubrum enabled the C-5 pathway in Rhodobacter sphaeroides to function upon assembling all the required genes 1.2.1.70 recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 1.2.1.70 wild-type GluTR and variants produced as N-terminal His6-fusion proteins