1.2.1.41 - 1.2.1.41 a fusion of the proA and proB gene of Bacillus subtilis, coding for gamma-glutamyl kinase and gamma-glutamyl phosphate reductase, are cloned for expression of an an artifical bifunctional enzyme in Escherichia coli JM83 1.2.1.41 artificial bifunctional enzyme, fusion of the genes proA and proB, resulting in enhanced intracellular concentrations of proline 1.2.1.41 into the pCR2.1 TOPO TA vector for sequencing 1.2.1.41 into the pGEM-T easy vector for sequencing 1.2.1.41 into the pGEM-TEasy plasmid for sequencing