1.2.1.32 - 1.2.1.32 expressed in Escherichia coli BL21(DE3) cells 1.2.1.32 expressed in Escherichia coli carrying pDTG912, pDTG912/A, and pDTG912/B 1.2.1.32 expressed in Escherichia coli HB101 1.2.1.32 expression as His-tag fusion protein in Escherichia coli Tuner (DE3) 1.2.1.32 gene dmpC 1.2.1.32 gene hpcC 1.2.1.32 gene phnG 1.2.1.32 gene xylG 1.2.1.32 gene xylG from TOL plasmid of strain mt-2 1.2.1.32 into the plasmid pDESTF1, which encodes an N-terminal six-His tag and is under control of the T7lac promoter, is used to transform Escherichia coli Tuner DE3 1.2.1.32 NAH plasmid of Pseudomonas putida, carrying the coding gene nahI, cloned into Escherichia coli 1.2.1.32 recombinant expressin of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli