1.2.1.12 - 287915, 287916, 287923, 287925, 287952, 287954, 655270, 684427, 695363, 698374, 698925, 725632 1.2.1.12 a 1008 bp DNA fragment from the plr-sdh-gapdh gene is ligated into the vector pGEX-6P-1 for expression of the protein in Escherichia coli BL212DE3 cells 674559 1.2.1.12 a 1200 bp fragment of the phosphorylating GAPC encoding cDNA is cloned in the antisense orientation under control of the CaMV 35S promoter into the plasmid pBinAR for transformation of Agrobacterium tumefaciens 675137 1.2.1.12 cloned into the pBluescript vector for expression in Escherichia coli GM-109 cells 672306 1.2.1.12 cloned into the pQE32 vector for expression in Escherichia coli M15 cells 674688 1.2.1.12 determination of complete nucleotide sequence of the coding as well as the noncoding flanking regions of the gene 287918 1.2.1.12 DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli 743394 1.2.1.12 DNA and amino acid sequence determination of isozyme uracil-DNA glycosylase, UDG 743329 1.2.1.12 expressed as a histidine fusion protein in Escherichia coli TOP 10 strain 656745 1.2.1.12 expressed in Escherichia coli 684972, 711617 1.2.1.12 expressed in Escherichia coli BL21(DE3) cells 724236, 724415, 725632 1.2.1.12 expressed in Escherichia coli BL21(DE3)-R3 cells 724223 1.2.1.12 expressed in Escherichia coli BL21(DE3)pLysS cells 689409 1.2.1.12 expressed in Escherichia coli JM109 cells 710790, 726190 1.2.1.12 expressed in Escherichia coli M15 cells 710757 1.2.1.12 expressed in Escherichia coli strain BL21(DE3) 687114 1.2.1.12 expressed in Escherichia coli strain W3CG and in HeLa cells 687602 1.2.1.12 expressed in Escherichia coli strainW3CG and in HeLa cells 687602 1.2.1.12 expressed in Escherichia coli TRG8 cells 686080 1.2.1.12 expressed in Escherichia coli XL1-blue cells transformed with pNeis2 688953 1.2.1.12 expressed in HEK-293T cells 688661 1.2.1.12 expressed in MCF-7 cells 684120 1.2.1.12 expressed in prostate cancer cell lines PC-3 and LNCaP 687584 1.2.1.12 expression in 293Tcells and HepG2.2.15 cells 695939 1.2.1.12 expression in COS-7 cell 698989 1.2.1.12 expression in COS-7 cells 684972 1.2.1.12 expression in Escherichia coli 287956, 679982, 695391, 697008, 697508, 698639, 699592, 724223, 740949 1.2.1.12 expression in INS-1 cells 696464 1.2.1.12 expression in sf21 cell 695902 1.2.1.12 expression of wild-type and mutant enzyme W84F in Escherichia coli 287947 1.2.1.12 FhGAPDH sequence comparisons, functional recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3) 741965 1.2.1.12 for expression in Escherichia coli BL21DE 673587 1.2.1.12 Gapdh gene is located on chromosome 14, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), addition of 36 amino acid residues (MGS SHHHHHHSSGLVPRGSHMASMTGGNNMGRGSGF-) at the N-terminus of rPfGapdh 743738 1.2.1.12 GAPDH gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 743740 1.2.1.12 gene cbbG, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 762770 1.2.1.12 gene CgGAP or GPD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, promoter analysis, six stress-response elements were found in the upstream region of the CgGAP gene. Recombinant expression in enzyme-deficient Saccharomyces cerevisiae from plasmid pYX212-CgGAP and functional complementation restoring the wild-type phenotype. The GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cerevisiae cells grown on glucose, determined via GFP-tagged gene expression analysis 741472 1.2.1.12 gene CgGAP, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, six stress-response elements are found in the upstream region of the CgGAP gene, phylogenetic analysis, funtional complementation of Saccharomyces cerevisiae transformed with CgGAP and restoration to the wild-type when cultured in high-osmolarity medium. Promoter studies in Saccharomyces cerevisiae using the GFP gene as a reporter show that the GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cells grown on D-glucose 743894 1.2.1.12 gene gap, recombinant expression of His-tagged wild-type and mutant enzymes in Mycobacterium tuberculosis strain H37Ra cell cytosol 763064 1.2.1.12 gene gapA, complementation of the mutant Escherichia coli strain MG1655DELTAgapA by the Streptococcus mutans gapN gene, EC 1.2.1.9, UniProt ID Q59931, expressed from plasmid pTrcgapN 741625 1.2.1.12 gene gapA, quantitative real-time PCR enzyme expression analysis, GAPDHS overexpression in melanoma cells (OCM1 cells), phenotype with and without SOX10 knockout, overview 763063 1.2.1.12 gene gapA, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 742668 1.2.1.12 gene gapA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 762577, 762579 1.2.1.12 gene gapA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) CodonPlus RIPL 762576 1.2.1.12 gene gapA, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 743222 1.2.1.12 gene gapA, sequence comparisons 742060 1.2.1.12 gene gapc, targeted expression of plastidial glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in a glyceraldehyde-3-phosphate dehydrogenase double mutant background gapcp1gapcp2 743542 1.2.1.12 gene gapdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha 763540 1.2.1.12 gene gapdh, recombinant expression in Escherichia coli 762842 1.2.1.12 gene GAPDH1, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant overexpression in Mortierella alpina using Agrobacterium tumefaciens-mediated transformation 763079 1.2.1.12 gene GAPDH2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant expression using Agrobacterium tumefaciens-mediated transformation 763079 1.2.1.12 gene GPD, quantitative RT-PCR enzyme expression analysis, molecular phylogenetic tree using sequences from 26 GAPDH proteins from 12 species of Aspergillus and 8 species of Trichoderma genus 762945 1.2.1.12 gene Rv1436, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli 741758 1.2.1.12 genome contains two unlinked genes for D-glyceraldehyde 3-phosphate dehydrogenase: one of them encodes a protein of 37000 Da and the other a protein of 35500 Da 287924 1.2.1.12 into a pGEX-5X-3 and a pGFP-C2 vector 676259 1.2.1.12 into the pET14b vector for expression in Escherichia coli BL21 Star cells 677046 1.2.1.12 into the pET15b vector for expression in Escherichia coli BL21DE3 cells 671063 1.2.1.12 into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells 663144 1.2.1.12 into the TOPO pET100 expression vector for transformation of Escherichia coli XL1-Blue competent cells 673986 1.2.1.12 nuclear-encoded, plastid specific enzyme 287922 1.2.1.12 recombinant enzyme expression 743295 1.2.1.12 recombinant enzyme expression of GST-tagged GAPDH in gapA-deficient Escherichia coli strain DS112 743295 1.2.1.12 recombinant enzyme expression of GST-tagged tGAPDHS (amino acids 76-408) in gapA-deficient Escherichia coli strain DS112 743295 1.2.1.12 recombinant enzyme expression of MBP-tagged tGAPDHS (amino acids 106-438) in gapA-deficient Escherichia coli strain DS112 743295 1.2.1.12 recombinant expression of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 has no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affects Arabidopsis thaliana development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Recombinant expression of GFP-tagged isozyme GAPCp1 in Arabidopsis thaliana, quantitative real-time-PCR expression analysis 743496 1.2.1.12 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Codon PlusR (DE3) RIL 763105 1.2.1.12 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 762943 1.2.1.12 recombinant expression of mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101 741963 1.2.1.12 recombinant expression of untagged enzyme in Escherichia coli strain Rosetta 2 (DE3) 763665 1.2.1.12 recombinant expression of wild-type and mutant enzymes carrying a 20-residue N-terminal tag containing hexa-histidine and a thrombin cleavage sequence in Escherichia coli strain Rosetta (DE3) pLysS 743613 1.2.1.12 recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101 742014 1.2.1.12 recombinant expression of wild-type and mutant enzymes in HEK-293 cells 741810 1.2.1.12 recombinant expression of wild-type enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain HB101 741963 1.2.1.12 recombinant expression of wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101 742014 1.2.1.12 sequence comparisons 742889 1.2.1.12 subcloned into a pET14b vector for expression in Escherichia coli BL21DE3 cells 671070 1.2.1.12 subcloned into the pET15b vector for expression in Escherichia coli cells 671066 1.2.1.12 the rat cDNA library is cloned in the target vector pTRG, Escherichia coli is used as a reporter strain 674503 1.2.1.12 two genes for glyceraldehyde-3-phosphate dehydrogenase 287912