1.14.99.64 expression in Escherichia coli 1.14.99.64 transgenic Arabidopsis thaliana expressing the beta-carotene ketolase from Chlamydomonas reinhardtii develop orange leaves which accumulate astaxanthin up to 2 mg/g dry weight with a 1.8fold increase in total carotenoids. The ketolases with high activity of converting zeaxanthin to astaxanthin triggers transgenic Arabidopsis to accumulate large amounts of astaxanthin in leaves and seeds