1.14.99.29	-	676855, 686708	
1.14.99.29	construction of a polycistronic vector encoding eIF5A, DHS and DOHH, expression in Escherichia coli strain BL21(DE3), overview. Effects of time and temperature on the expression of recombinant proteins and hypusine modification, DOHH effectively hydroxylates all the eIF5A(Dhp), a deoxyhypusine-containing intermediate, formed	716821	
1.14.99.29	DNA and amino acid sequence determination and analysis, different clones, sequence comparisons, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109	689862	
1.14.99.29	expressed in Escherichia coli BL21 cells	744002	
1.14.99.29	expressed in Escherichia coli BL21(DE3) cells	746515	
1.14.99.29	expressed in Escherichia coli BL21(DE3)pLysS cells	745386	
1.14.99.29	expressed in Escherichia coli BL21-CodonPlus (DE3) cells	744989	
1.14.99.29	expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	674901	
1.14.99.29	for expression in Escherichia coli cells	700997	
1.14.99.29	gene dohh	713727	
1.14.99.29	gene dohh, cloned from a GST-ORF library in a biochemical genomics approach. The dohh gene complements a yeast deficient mutant YJR070C, expression as GST-tagged protein	713727	
1.14.99.29	gene dohh, quantitative expression analysis	727927	
1.14.99.29	gene DOHH, quantitative real-time PCR expression analysis	715197	
1.14.99.29	gene dohh, sequence comparison, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	728594	
1.14.99.29	gene LIA1, expression in Escherichia coli strain BL21(DE3) as GST-tagged protein	713728	
1.14.99.29	into the pST-Acceptor vector for sequencing, into the vectors pET-15b and pET-28a for expression in Escherichia coli BL21DE3 cells	697912	
1.14.99.29	single copy gene dohh	701650, 713727	
1.14.99.29	single copy gene dohh, expression as GST-tagged protein, expression of the hypusine pathway genes in Escherichia coli as His-tagged proteins	713727