1.14.20.5 - 1.14.20.5 expressed in Escherichia coli BLR (DE3) cells 1.14.20.5 expressed in Escherichia coli strain BL21Star 1.14.20.5 expressed in Saccharomyces cerevisiae 1.14.20.5 expression in Arabidopsis thaliana 1.14.20.5 expression in Escherichia coli 1.14.20.5 expression in Saccharomyces cerevisiae 1.14.20.5 expression in yeast 1.14.20.5 gene DvFNS, real-time RT-PCR expression analysis 1.14.20.5 RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli 1.14.20.5 the open reading frame of the flavone synthase gene PFNS-1 is cloned from Populus deltoids using RT-PCR, subcloned into Escherichia coli expression vector pGEX 5X-2, the flavone 7-O-methyltransferase into pRSF-Duet vector, both cotransformed into Escherichia coli BL21