1.14.13.29	-	
1.14.13.29	DNA and amino acid sequence determination and analysis, phylogenetic analysis	
1.14.13.29	PCR-amplification, shuttle vector pK4 is used to construct pKPN plasmids containing enzyme gene and reporter gene, hosts are Rhodococcus sp. PN1 and Rhodococcus rhodochrous ATCC 12674 (electroporation), Escherichia coli JM109 is host for propagation and purification of recombinant plasmids	
1.14.13.29	recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)/pLysS