1.14.13.163 DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 746248 1.14.13.163 expression in Escherichia coli 690527 1.14.13.163 gene hspB, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 745310 1.14.13.163 gene vppD cloned from a 97-kbp DNA fragment containing six nicotine degradation-related genes which is obtained by gap closing from the genome sequence of strain SJY1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 741691 1.14.13.163 recombinant expression of His-tagged enzyme in Escherichia coli strain B21-Codon Plus(DE3)-RIL 744640