1.13.12.5 catalytic domain (19 kDa) is expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins 1.13.12.5 expressed as a soluble form in Escherichia coli BL21(DE3)pLysS (Novagen) containing the expression vector pET-21a inserted with cDNA of Renilla luciferase 1.13.12.5 expressed in Escherichia coli 1.13.12.5 expressed in HEK-293T cells 1.13.12.5 expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins 1.13.12.5 expression in 293T HEK fibroblasts and HF8 human fibroblasts 1.13.12.5 expression in Arabidopsis 1.13.12.5 expression in Chlamydomonas reinhardtii 1.13.12.5 expression in CHO cells and HepG2 cells 1.13.12.5 expression in CHO-K1 cells and PC12D cells 1.13.12.5 expression in Escherichia coli 1.13.12.5 expression in Escherichia coli BL21 1.13.12.5 expression in Escherichia coli BL21 Star (DE3) 1.13.12.5 expression in Escherichia coli DH10B cells and 293T human kidney fibroblasts 1.13.12.5 expression in Escherichia coli DH5alpha 1.13.12.5 expression in Escherichia coli strain BL21 1.13.12.5 expression in Escherichia coli strain DH5alpha and MCF-7 cells 1.13.12.5 expression in Escherichia coli strain ER 2566 1.13.12.5 expression in HEK-293 cells or Grip-Tite 293 MSR cells 1.13.12.5 expression in HeLa-cell 1.13.12.5 expression in Hep-G2 cell 1.13.12.5 expression in insect cells using a baculovirus expression system 1.13.12.5 expression in LNCaP cell, pRL-SV40 cell, pRL-null cell 1.13.12.5 expression in mouse and human C57/BL6 primary T cells, expression in PG-13 cells 1.13.12.5 expression in rabbit leukocyte lysate 1.13.12.5 expression of His-tagged apoclytin into the periplasmic space in Escherichia coli 1.13.12.5 expression of Renilla luciferase split fragments in HeLa cells, mitochondrial localization, overview 1.13.12.5 expression of RLuc8 in Escherichia coli BL21 1.13.12.5 expression of stabilized variant RLuc8 with noncleavable N-terminal His6-tag 1.13.12.5 fragments of Renilla luciferase (Rluc) are fused to the chemotaxis-associated response regulator CheY3 and its phosphatase CheZ in the enteric pathogen Vibrio cholerae in order to demonstrate dynamic protein-protein interactions by luciferase complementation 1.13.12.5 gene luc, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 1.13.12.5 recombinant enzyme expression in ES-2 cells 1.13.12.5 recombinant expression of engineered His-tagged enzyme C-SRLuc8 in Escherichia coli, method optimization 1.13.12.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 1.13.12.5 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) 1.13.12.5 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 1.13.12.5 recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 1.13.12.5 recombinant expression of soluble His6-tagged enzyme FcUni-RLuc in Escherichia coli strain BL21 (DE3), an Fc-binding peptide is separated by a five-amino acid linker from RLuc